![]() Grab everything! You do that, and you just saved yourself 20 Plasma cores in making them in the future…… Hehehehe yeah □ the second way to make these Polymerized Plasma cores is to use Plasma cores! □ So it's basically just plasma cores crunched together then colored blue.īut anyways, let’s not talk about what the rest of you don’t have and discuss what we all have in common, Basic resources. So, to those of you who don't need Polymerized cores right now, COLLECT THEM WHEN THEIR UP FOR GRABS!!! Every time the Plasma leakage event appears. Now that I am Plasma level 5 and have now access to creating the devil ingredient that made Plasma level 3 into the new HQ level 30, I have to face yet another resource that is difficult to obtain, as if collecting Plasma cores wasn’t enough. Even for once in a blue moon spender like me who takes advantage of any great deal and hoard all the stuff that I can on a daily basis. The phenylalanine concentrations will be measured by HPLC analysis of supernatants from trichloroacetic acid extracts of plasma.The journey to Plasma level 4-5 is a long one even with the new events rewarding more Plasma cores and the updated intel missions. Collected blood will be used for determination of phenylalanine concentrations and radioactivity. At 15-min following the injection, the mouse will be anesthetized, and samples will be collected. L-phenylalanine (0.2 ♜i/ml/µmol, 30 ♜i/100 g/body wt 1 ml/100 g/body wt) will be intravenously bolus injected into awake mice. In vivo rates of protein synthesis in individual organs (heart, skeletal muscle, liver, adipose tissue, lung, and kidney) will be measured in awake mice by determining the amount of L-Phenylalanine incorporation into proteins. Organ-specific triglyceride levels will be measured using spectrophometry, and -labeled triglyceride will be measured using liquid scintillation Protein metabolism in individual organs in conscious mice Briefly, tissues will be homogenized with chloroform/methanol, and lipid layer will be extracted using H2SO4. At the end of experiment, tissue samples will be taken to measure palmitate incorporation into organ-specific triglyceride. Additional blood samples will be taken at the end to measure plasma fatty acids levels. Blood samples will be collected at 0, 0.5, 1, 2, 3, 4, and 5 min following the injection for the measurement of plasma palmitate concentrations. Palmitate (10 ♜i) will be intravenously injected to assess basal lipid uptake into individual organs. Lipid metabolism in individual organs in conscious mice Basal whole body and organ-specific glucose metabolism will be calculated as described in the Hyperinsulinemic-euglycemic clamp study. At the end of experiment, mice will be anesthetized, and tissue samples will be taken for organ-specific levels of 2-deoxyglucose-6-phosphate. Basal glucose uptake in individual organs will be measured using an intravenous injection of 2-deoxyglucose and taking blood samples at 5-min intervals for 30 min to determine plasma 2-deoxyglucose concentrations. Glucose metabolism in individual organs in conscious miceįollowing overnight fast or fed state (5-hr after food removal), glucose (0.05 ♜i/min) will be intravenously infused for 2 hrs in awake mice to assess basal whole body glucose metabolism.
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